Hotspot

trnP-GGG-psaJ

LSC · 66383-66772 · 390 bp

Back to Temochloa

Pi 0.00684

MarkerSeek score 50.50

Primer available yes

Label trnP-GGG-psaJ

Feature Metadata

label_name: trnP-GGG-psaJ
feature_type: igs
region: LSC
length_bp: 390
variable_sites: 4
pi: 0.00684
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-psaJ_p1	AAAACAAACGCGCTACCAAG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.9	57.9	498 bp	1.0	79.0
trnP-GGG-psaJ_p2	CAAAACAAACGCGCTACCAA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.2	57.9	499 bp	1.0	78.5
trnP-GGG-psaJ_p3	TTGTACCCAAAACAAACGCG	AAACCTGCTAAAGCCCCAAA	45.0	45.0	57.8	57.9	506 bp	1.0	78.3
trnP-GGG-psaJ_p4	TACCCAAAACAAACGCGCTA	AAACCTGCTAAAGCCCCAAA	45.0	45.0	58.4	57.9	503 bp	1.0	76.6
trnP-GGG-psaJ_p5	AAAACAAACGCGCTACCAAG	CTGCTAAAGCCCCAAACCAT	45.0	50.0	57.9	58.4	494 bp	1.0	76.2