Hotspot

rbcL-psaI

LSC · 58013-59265 · 1253 bp

Back to Temochloa

Pi 0.00642

MarkerSeek score 62.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1253
variable_sites: 12
pi: 0.00642
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CGCAGCTTGTGAAGTATGGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	1383-1390 bp	1.0	78.8
rbcL-psaI_p2	CGCAGCTTGTGAAGTATGGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.3	57.9	1389-1396 bp	1.0	78.2
rbcL-psaI_p3	GGCGATCAAATTCGAGTTCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.6	58.0	1362-1369 bp	1.0	78.2
rbcL-psaI_p4	TGGAAGGCGATCAAATTCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.6	58.0	1367-1374 bp	1.0	77.8
rbcL-psaI_p5	GGCGATCAAATTCGAGTTCG	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.6	57.9	1368-1375 bp	1.0	77.6