Hotspot

trnG-UCC-trnT-GGU

LSC · 12524-15933 · 3410 bp

Back to Temochloa

Pi 0.00518
MarkerSeek score 58.80
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
3410
variable_sites
26
pi
0.00518
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 TGCCCCTTTGGTCTTTTGTT CGTGAAGAGAGTTGACACACT 45.0 47.6 58.1 57.9 248 bp 0.666667 57.0
trnG-UCC-trnT-GGU_p2 TTGCCCCTTTGGTCTTTTGT CGTGAAGAGAGTTGACACACT 45.0 47.6 58.1 57.9 249 bp 0.666667 57.0
trnG-UCC-trnT-GGU_p3 GCTCTTATTGCCCCTTTGGT CGTGAAGAGAGTTGACACACT 50.0 47.6 58.1 57.9 256 bp 0.666667 56.8
trnG-UCC-trnT-GGU_p4 AACAGAAAACCCATAGCCGG CGTGAAGAGAGTTGACACACT 50.0 47.6 58.2 57.9 831-852 bp 0.666667 56.8
trnG-UCC-trnT-GGU_p5 TTTTTGTTGGGCAGGCAGTA CGTGAAGAGAGTTGACACACT 45.0 47.6 58.2 57.9 778-799 bp 0.666667 56.3