Hotspot

psbK-psbI

LSC · 7272-7670 · 399 bp

Back to Temochloa

Pi 0.015

MarkerSeek score 64.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 9
pi: 0.015
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGTGGATGTTATGCCTGTCA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.0	57.8	592 bp	1.0	79.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTACTCCTCACGCCCAG	50.0	50.0	57.9	57.8	543 bp	1.0	79.4
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	540 bp	1.0	77.4
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	541 bp	1.0	77.4
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TTTTTACTCCTCACGCCCAG	45.0	50.0	58.3	57.8	542 bp	1.0	77.4