Hotspot

rps12-rrn16

Genome · 135961-137860 · 1900 bp

Back to Tectaria

Pi 0.105

MarkerSeek score 93.30

Primer available yes

Label rps12-rrn16

Feature Metadata

label_name: rps12-rrn16
feature_type: igs
region: Genome
length_bp: 1900
variable_sites: 199
pi: 0.105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 93.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rrn16_p1	GAACTCTCGCGACTTTACGT	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.0	57.9	2025 bp	0.5	58.9
rps12-rrn16_p2	CTCTCGCGACTTTACGTAGG	TCCGACTTGCATGTGTTAGG	55.0	50.0	57.9	57.9	2022 bp	0.5	58.5
rps12-rrn16_p3	AGCCGTTACCTCAAACTTGG	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	2054 bp	0.5	58.4
rps12-rrn16_p4	GCCGTTACCTCAAACTTGGA	TCCGACTTGCATGTGTTAGG	50.0	50.0	58.1	57.9	2053 bp	0.5	58.3
rps12-rrn16_p5	GGTTAATCGAACTCTCGCGA	TCCGACTTGCATGTGTTAGG	50.0	50.0	57.8	57.9	2033 bp	0.5	57.8