Hotspot

psaC-ndhE

Genome · 111098-111419 · 322 bp

Back to Tectaria

Pi 0.0946

MarkerSeek score 80.10

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: Genome
length_bp: 322
variable_sites: 30
pi: 0.0946
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CCTCACACACTGGGTACAAC	GCCCTTTCTCTCCAACGAAA	55.0	50.0	58.1	58.1	443-445 bp	1.0	84.5
psaC-ndhE_p2	CTCACACACTGGGTACAACC	GCCCTTTCTCTCCAACGAAA	55.0	50.0	58.1	58.1	442-444 bp	1.0	84.5
psaC-ndhE_p3	CCTCACACACTGGGTACAAC	ATTGCCCTTTCTCTCCAACG	55.0	50.0	58.1	58.2	446-448 bp	1.0	83.9
psaC-ndhE_p4	CTCACACACTGGGTACAACC	ATTGCCCTTTCTCTCCAACG	55.0	50.0	58.1	58.2	445-447 bp	1.0	83.9
psaC-ndhE_p5	TCTGATTTGCCTTGCATCCA	GCCCTTTCTCTCCAACGAAA	45.0	50.0	57.8	58.1	501-503 bp	1.0	83.0