Hotspot

psbF-psbE

Genome · 61206-61220 · 15 bp

Back to Tectaria

Pi 0.0667

MarkerSeek score 83.30

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: Genome
length_bp: 15
variable_sites: 1
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	AGCTGATATGGACCCCAAGA	GAGAGCCGACAAGAAGTTCC	50.0	55.0	57.8	58.3	195 bp	1.0	76.6
psbF-psbE_p2	AGCTGATATGGACCCCAAGA	TCAGAGAGCCGACAAGAAGT	50.0	50.0	57.8	58.4	198 bp	1.0	75.8
psbF-psbE_p3	AGCTGATATGGACCCCAAGA	AGTTCCCCTAGTAACTGGCC	50.0	55.0	57.8	58.4	181 bp	1.0	75.5
psbF-psbE_p4	AGCTGATATGGACCCCAAGA	CAGAGAGCCGACAAGAAGTT	50.0	50.0	57.8	57.6	197 bp	1.0	75.3
psbF-psbE_p5	GCATAGCTGATATGGACCCC	GAGAGCCGACAAGAAGTTCC	55.0	55.0	57.6	58.3	199 bp	1.0	74.9