Hotspot

ndhC-trnV-UAC

Genome · 48574-49075 · 502 bp

Back to Tectaria

Pi 0.0828

MarkerSeek score 81.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 502
variable_sites: 40
pi: 0.0828
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGAGTCATATTGTTGGAGCGA	CCAAGCAGTTCAGGAAGGTT	47.6	50.0	57.8	58.0	592-609 bp	1.0	78.3
ndhC-trnV-UAC_p2	AGGAGTCATATTGTTGGAGCG	CCAAGCAGTTCAGGAAGGTT	47.6	50.0	57.8	58.0	593-610 bp	1.0	78.3
ndhC-trnV-UAC_p3	GGAGTCATATTGTTGGAGCGA	CGATCTGCATGACCGAGAAT	47.6	50.0	57.8	57.9	560-577 bp	1.0	77.7
ndhC-trnV-UAC_p4	AGGAGTCATATTGTTGGAGCG	CGATCTGCATGACCGAGAAT	47.6	50.0	57.8	57.9	561-578 bp	1.0	77.6
ndhC-trnV-UAC_p5	GGAGTCATATTGTTGGAGCGA	CCGAGAATGTCTACGGTTCG	47.6	55.0	57.8	58.2	548-565 bp	1.0	77.6