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rps4-trnL-UAA

Genome · 45017-45627 · 611 bp

Back to Tectaria

Pi 0.0869

MarkerSeek score 83.30

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 611
variable_sites: 53
pi: 0.0869
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	TCAGTTTCAAACGAGGTCCTC	GGCTGCCACTTGATATGTCT	47.6	50.0	57.9	57.7	722-733 bp	1.0	74.4
rps4-trnL-UAA_p2	TCAGTTTCAAACGAGGTCCTC	CTACCATTCCGCCATATCCC	47.6	55.0	57.9	57.6	666-677 bp	1.0	74.3
rps4-trnL-UAA_p3	TCAGTTTCAAACGAGGTCCT	GGCTGCCACTTGATATGTCT	45.0	50.0	56.7	57.7	722-733 bp	1.0	72.9
rps4-trnL-UAA_p4	TCAGTTTCAAACGAGGTCCT	CTACCATTCCGCCATATCCC	45.0	55.0	56.7	57.6	666-677 bp	1.0	72.9
rps4-trnL-UAA_p5	TCAGTTTCAAACGAGGTCCTC	GGGCTGCCACTTGATATGTC	47.6	55.0	57.9	58.7	723-734 bp	1.0	71.6