Hotspot

trnS-GGA-rps4

Genome · 43939-44395 · 457 bp

Back to Tectaria

Pi 0.0544

MarkerSeek score 78.60

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 457
variable_sites: 24
pi: 0.0544
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TAGAGTTCCAGCTACCGAGG	AGTATTACTCCCGCAAAGCC	55.0	50.0	57.9	57.7	520-521 bp	1.0	83.1
trnS-GGA-rps4_p2	TAGAGTTCCAGCTACCGAGG	GTATTACTCCCGCAAAGCCT	55.0	50.0	57.9	57.7	519-520 bp	1.0	83.1
trnS-GGA-rps4_p3	TAGAGTTCCAGCTACCGAGG	GCCAATCGAGAATCCGTCAA	55.0	50.0	57.9	58.3	569-570 bp	1.0	82.4
trnS-GGA-rps4_p4	CTACCGAGGGTTCAAATCCC	GTATTACTCCCGCAAAGCCT	55.0	50.0	57.7	57.7	508-509 bp	1.0	81.1
trnS-GGA-rps4_p5	CTACCGAGGGTTCAAATCCC	AGTATTACTCCCGCAAAGCC	55.0	50.0	57.7	57.7	509-510 bp	1.0	81.0