Hotspot

trnC-GCA-trnG-GCC

Genome · 29999-30490 · 492 bp

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Pi 0.124
MarkerSeek score 79.80
Primer available yes
Label trnC-GCA-trnG-GCC

Feature Metadata

label_name
trnC-GCA-trnG-GCC
feature_type
igs
region
Genome
length_bp
492
variable_sites
61
pi
0.124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-trnG-GCC_p1 GATTTGCAGTCCTCCGTCTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.9 58.2 552-588 bp 1.0 77.8
trnC-GCA-trnG-GCC_p2 GATTTGCAGTCCTCCGTCTT GGGTTCGATTCCCGCTATC 50.0 57.9 57.9 57.8 554-590 bp 1.0 77.6
trnC-GCA-trnG-GCC_p3 GATTTGCAGTCCTCCGTCTT GGTTCGATTCCCGCTATCC 50.0 57.9 57.9 57.8 553-589 bp 1.0 77.6
trnC-GCA-trnG-GCC_p4 TCCTCCGTCTTACCACTTGA GTTCGATTCCCGCTATCCG 50.0 57.9 57.7 58.2 543-579 bp 1.0 76.2
trnC-GCA-trnG-GCC_p5 AGTCCTCCGTCTTACCACTT GTTCGATTCCCGCTATCCG 50.0 57.9 57.7 58.2 545-581 bp 1.0 76.2