Hotspot

psbM-petN

Genome · 28228-29374 · 1147 bp

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Pi 0.0947
MarkerSeek score 84.00
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
Genome
length_bp
1147
variable_sites
108
pi
0.0947
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 ACCGCACCTTTCATTCCAAT CTGCTCTGACGGTGATTTCC 45.0 55.0 58.1 58.6 1287-1308 bp 1.0 81.3
psbM-petN_p2 TTGTAGCTACCGCACCTTTC CTGCTCTGACGGTGATTTCC 50.0 55.0 57.9 58.6 1295-1316 bp 1.0 81.2
psbM-petN_p3 CTACCGCACCTTTCATTCCA CTGCTCTGACGGTGATTTCC 50.0 55.0 57.9 58.6 1289-1310 bp 1.0 81.1
psbM-petN_p4 CCAATCCCGACAGCTTTTCT CTGCTCTGACGGTGATTTCC 50.0 55.0 58.2 58.6 1272-1293 bp 1.0 80.6
psbM-petN_p5 TCCAATCCCGACAGCTTTTC CTGCTCTGACGGTGATTTCC 50.0 55.0 58.2 58.6 1273-1294 bp 1.0 80.6