Hotspot

trnD-GUC-trnY-GUA

Genome · 27467-27518 · 52 bp

Back to Tectaria

Pi 0.115

MarkerSeek score 82.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 52
variable_sites: 6
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GTTCGAGACCCGTCAATCC	ACTTCTCACTTCTGGGTCGA	57.9	50.0	58.0	58.0	168 bp	1.0	74.5
trnD-GUC-trnY-GUA_p2	GGTTCGAGACCCGTCAATC	ACTTCTCACTTCTGGGTCGA	57.9	50.0	58.0	58.0	169 bp	1.0	74.4
trnD-GUC-trnY-GUA_p3	AGTTCAATCGGTTAGAGCACC	ACTTCTCACTTCTGGGTCGA	47.6	50.0	58.0	58.0	213 bp	1.0	74.0
trnD-GUC-trnY-GUA_p4	GTTCGAGACCCGTCAATCC	CACTTCTCACTTCTGGGTCG	57.9	55.0	58.0	57.9	169 bp	1.0	73.9
trnD-GUC-trnY-GUA_p5	AGTTCAATCGGTTAGAGCACC	CACTTCTCACTTCTGGGTCG	47.6	55.0	58.0	57.9	214 bp	1.0	73.5