Hotspot

chlL-trnH-GUG

Genome · 127852-128058 · 207 bp

Back to Taxus

Pi 0.049
MarkerSeek score 73.70
Primer available yes
Label chlL-trnH-GUG

Feature Metadata

label_name
chlL-trnH-GUG
feature_type
igs
region
Genome
length_bp
207
variable_sites
30
pi
0.049
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlL-trnH-GUG_p1 TCAATGGAGCAGCAAAACCT TGACCATTCAGCCATGGATG 45.0 50.0 58.0 57.9 1008-1326 bp 0.909091 80.7
chlL-trnH-GUG_p2 TCAATGGAGCAGCAAAACCT TGCTTTGACCATTCAGCCAT 45.0 45.0 58.0 58.1 1013-1331 bp 0.909091 80.6
chlL-trnH-GUG_p3 TCAATGGAGCAGCAAAACCT CCGCGACTTCCCAATTATGA 45.0 50.0 58.0 58.1 1039-1254 bp 0.818182 76.5
chlL-trnH-GUG_p4 TCAATGGAGCAGCAAAACCT TTGGGTGCTTTGACCATTCA 45.0 45.0 58.0 57.9 1018-1233 bp 0.818182 76.4
chlL-trnH-GUG_p5 TCAATGGAGCAGCAAAACCT AGTTGGGTGCTTTGACCATT 45.0 45.0 58.0 57.9 1020-1235 bp 0.818182 76.3