Hotspot

ycf1-chlN

Genome · 124500-125583 · 1084 bp

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Pi 0.0356
MarkerSeek score 73.70
Primer available yes
Label ycf1-chlN

Feature Metadata

label_name
ycf1-chlN
feature_type
igs
region
Genome
length_bp
1084
variable_sites
107
pi
0.0356
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.85
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-chlN_p1 GCCATTGTATGAGGCTTCCA TGGAATGGCTCATGCTAACC 50.0 50.0 57.9 57.9 1389-1655 bp 1.0 89.6
ycf1-chlN_p2 GGCGGTACTTAGCAGAAACA TGGAATGGCTCATGCTAACC 50.0 50.0 57.9 57.9 1630-1896 bp 1.0 88.7
ycf1-chlN_p3 GCCATTGTATGAGGCTTCCA TGTTGAAACCCAAGGTCTGG 50.0 50.0 57.9 57.9 1716-1982 bp 1.0 88.4
ycf1-chlN_p4 GCCATTGTATGAGGCTTCCA GGTGTTGAAACCCAAGGTCT 50.0 50.0 57.9 57.9 1718-1984 bp 1.0 88.4
ycf1-chlN_p5 GGCGGTACTTAGCAGAAACA TGTTGAAACCCAAGGTCTGG 50.0 50.0 57.9 57.9 1957-2223 bp 1.0 87.8