Hotspot

ndhF-rpl32

Genome · 105737-106547 · 811 bp

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Pi 0.00589
MarkerSeek score 69.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
Genome
length_bp
811
variable_sites
17
pi
0.00589
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GAAAGCGGAAAGAAAGGCAC GCCCGATATGCTTTTCCCTT 50.0 50.0 57.9 58.3 866-956 bp 1.0 82.6
ndhF-rpl32_p2 GAAAGCGGAAAGAAAGGCAC CTTCCAAACATTGCTGCGAA 50.0 45.0 57.9 57.6 849-939 bp 1.0 81.7
ndhF-rpl32_p3 GAAAGCGGAAAGAAAGGCAC TACGCTTCTTTGGAACTGCC 50.0 50.0 57.9 58.5 808-898 bp 1.0 81.3
ndhF-rpl32_p4 AAAGAAAGGCACGATCCGAG GCCCGATATGCTTTTCCCTT 50.0 50.0 58.3 58.3 858-948 bp 1.0 80.8
ndhF-rpl32_p5 GAAAGAAAGGCACGATCCGA GCCCGATATGCTTTTCCCTT 50.0 50.0 58.3 58.3 859-949 bp 1.0 80.8