Hotspot

CD863_pgr002-CD863_pgr001

Genome · 101889-102103 · 215 bp

Back to Taxus

Pi 0.0258
MarkerSeek score 78.30
Primer available yes
Label CD863_pgr002-CD863_pgr001

Feature Metadata

label_name
CD863_pgr002-CD863_pgr001
feature_type
igs
region
Genome
length_bp
215
variable_sites
16
pi
0.0258
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
CD863_pgr002-CD863_pgr001_p1 GCTCTCCATAAGGTCAAGGC AGTATCGTCACCGCAGTAGA 55.0 50.0 58.0 58.0 371-561 bp 1.0 86.8
CD863_pgr002-CD863_pgr001_p2 GCTCTCCATAAGGTCAAGGC TATCGTCACCGCAGTAGAGT 55.0 50.0 58.0 58.0 369-559 bp 1.0 86.8
CD863_pgr002-CD863_pgr001_p3 GCTCTCCATAAGGTCAAGGC GCATCGAGCTATTTTTCCGC 55.0 50.0 58.0 58.0 405-595 bp 1.0 86.7
CD863_pgr002-CD863_pgr001_p4 CCATAAGGTCAAGGCGAGAC TATCGTCACCGCAGTAGAGT 55.0 50.0 58.1 58.0 364-554 bp 1.0 86.7
CD863_pgr002-CD863_pgr001_p5 CCATAAGGTCAAGGCGAGAC GCATCGAGCTATTTTTCCGC 55.0 50.0 58.1 58.0 400-590 bp 1.0 86.5