Hotspot

trnV-GAC-CD863_pgr004

Genome · 94691-95187 · 497 bp

Back to Taxus

Pi 0.0232
MarkerSeek score 78.00
Primer available yes
Label trnV-GAC-CD863_pgr004

Feature Metadata

label_name
trnV-GAC-CD863_pgr004
feature_type
igs
region
Genome
length_bp
497
variable_sites
40
pi
0.0232
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-CD863_pgr004_p1 CCGAATGAGGTTAGGGAAGG AAACACCACTTCACGTTCGA 55.0 45.0 57.4 58.0 580-703 bp 1.0 84.2
trnV-GAC-CD863_pgr004_p2 CGAATGAGGTTAGGGAAGGG AAACACCACTTCACGTTCGA 55.0 45.0 57.4 58.0 579-702 bp 1.0 84.2
trnV-GAC-CD863_pgr004_p3 CCGAATGAGGTTAGGGAAGG CCACTGGAAACACCACTTCA 55.0 50.0 57.4 57.9 587-710 bp 1.0 83.8
trnV-GAC-CD863_pgr004_p4 CGAATGAGGTTAGGGAAGGG CCACTGGAAACACCACTTCA 55.0 50.0 57.4 57.9 586-709 bp 1.0 83.8
trnV-GAC-CD863_pgr004_p5 CGAATGAGGTTAGGGAAGGG TGGAAACACCACTTCACGTT 55.0 45.0 57.4 57.9 582-705 bp 1.0 83.3