Hotspot

accD-infA

Genome · 54711-56590 · 1880 bp

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Pi 0.0336
MarkerSeek score 72.60
Primer available yes
Label accD-infA

Feature Metadata

label_name
accD-infA
feature_type
igs
region
Genome
length_bp
1880
variable_sites
168
pi
0.0336
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.92
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-infA_p1 TTCTCGTATGTGCTTCTGGC ACAAATGACCAGAAGGCGTT 50.0 45.0 58.0 58.0 2511-2710 bp 1.0 89.6
accD-infA_p2 GTATGGGCTCGGTAGTAGGT AACAAATGACCAGAAGGCGT 55.0 45.0 58.0 58.0 2584-2783 bp 1.0 89.5
accD-infA_p3 GTATGGGCTCGGTAGTAGGT ACAAATGACCAGAAGGCGTT 55.0 45.0 58.0 58.0 2583-2782 bp 1.0 89.5
accD-infA_p4 TATGGGCTCGGTAGTAGGTG AACAAATGACCAGAAGGCGT 55.0 45.0 58.0 58.0 2583-2782 bp 1.0 89.5
accD-infA_p5 TATGGGCTCGGTAGTAGGTG ACAAATGACCAGAAGGCGTT 55.0 45.0 58.0 58.0 2582-2781 bp 1.0 89.5