Hotspot

atpF-atpA

Genome · 38841-38933 · 93 bp

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Pi 0.0354
MarkerSeek score 82.50
Primer available yes
Label atpF-atpA

Feature Metadata

label_name
atpF-atpA
feature_type
igs
region
Genome
length_bp
93
variable_sites
10
pi
0.0354
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpA_p1 GCCTGCTTAGAGCCATGAAA AGCAATGCCATCTCCTACTTG 50.0 47.6 58.2 58.1 222-268 bp 1.0 77.3
atpF-atpA_p2 GCCTGCTTAGAGCCATGAAA GCAATGCCATCTCCTACTTGA 50.0 47.6 58.2 58.1 221-267 bp 1.0 77.3
atpF-atpA_p3 AATATCGGCCTGCTTAGAGC AGCAATGCCATCTCCTACTTG 50.0 47.6 57.5 58.1 229-275 bp 1.0 75.5
atpF-atpA_p4 AATATCGGCCTGCTTAGAGC GCAATGCCATCTCCTACTTGA 50.0 47.6 57.5 58.1 228-274 bp 1.0 75.5
atpF-atpA_p5 GCCTGCTTAGAGCCATGAAA AGCAATGCCATCTCCTACTT 50.0 45.0 58.2 56.6 222-268 bp 1.0 74.6