Hotspot

psbD-trnT-GGU

Genome · 19716-20853 · 1138 bp

Back to Taxus

Pi 0.0045
MarkerSeek score 69.00
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
Genome
length_bp
1138
variable_sites
21
pi
0.0045
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 ATAGACCAGACCAACCCACA GTGGTAGAGTAACGCCATGG 50.0 55.0 58.0 58.1 1280-1470 bp 1.0 84.5
psbD-trnT-GGU_p2 ATAGACCAGACCAACCCACA TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.0 58.1 1283-1473 bp 1.0 84.3
psbD-trnT-GGU_p3 TAGACCAGACCAACCCACAA GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1279-1469 bp 1.0 83.2
psbD-trnT-GGU_p4 TAGACCAGACCAACCCACAA TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1282-1472 bp 1.0 83.1
psbD-trnT-GGU_p5 ACAAAACGGTCTCTGCGTAG GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1257-1447 bp 1.0 83.0