Hotspot

cemA

Genome · 97344-98129 · 786 bp

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Pi 0.00127

MarkerSeek score 59.50

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: Genome
length_bp: 786
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TTCTCAGTCGGAACAAACCA	AGAGGGGAGGTAAAATGGGG	45.0	55.0	57.0	58.4	902 bp	1.0	69.0
cemA_p2	TTCTCAGTCGGAACAAACCA	GGGAGGTAAAATGGGGGAGA	45.0	55.0	57.0	58.4	898 bp	1.0	69.0
cemA_p3	TCTCAGTCGGAACAAACCATC	AGAGGGGAGGTAAAATGGGG	47.6	55.0	58.0	58.4	901 bp	1.0	68.6
cemA_p4	TCTCAGTCGGAACAAACCATC	GGGAGGTAAAATGGGGGAGA	47.6	55.0	58.0	58.4	897 bp	1.0	68.6
cemA_p5	CTCAGTCGGAACAAACCATCT	AGAGGGGAGGTAAAATGGGG	47.6	55.0	58.0	58.4	900 bp	1.0	68.6