Hotspot

psbD-trnE-UUC

Genome · 20994-22505 · 1512 bp

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Pi 0.00198

MarkerSeek score 59.90

Primer available yes

Label psbD-trnE-UUC

Feature Metadata

label_name: psbD-trnE-UUC
feature_type: igs
region: Genome
length_bp: 1512
variable_sites: 3
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnE-UUC_p1	AAGCAATAGACCGGACCAAC	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	57.9	57.8	1675 bp	1.0	77.9
psbD-trnE-UUC_p2	ACAAAACGGTCTCTGCGTAG	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.2	57.8	1647 bp	1.0	77.0
psbD-trnE-UUC_p3	AACAAAACGGTCTCTGCGTA	CGTTGCCTCCTTGAAAGAGA	45.0	50.0	57.8	57.8	1648 bp	1.0	77.0
psbD-trnE-UUC_p4	AAGCAATAGACCGGACCAAC	GAAAGAGAGATGTCCTGGGC	50.0	55.0	57.9	57.7	1663 bp	1.0	76.7
psbD-trnE-UUC_p5	ACAAAACGGTCTCTGCGTAG	GAAAGAGAGATGTCCTGGGC	50.0	55.0	58.2	57.7	1635 bp	1.0	75.8