Hotspot

infA

LSC · 65961-66197 · 237 bp

Back to Taxillus

Pi 0.0344

MarkerSeek score 64.40

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 237
variable_sites: 28
pi: 0.0344
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	ATGACGTTTTCTGCTGTGGT	AATGACAGACCGAGAAGCTC	45.0	50.0	58.0	57.3	670-1100 bp	1.0	86.4
infA_p2	AATGCTGAATCTCTGCCGAG	AATGACAGACCGAGAAGCTC	50.0	50.0	58.0	57.3	751-1178 bp	1.0	86.3
infA_p3	CAGCAAATGGTGTCCCTCTT	AATGACAGACCGAGAAGCTC	50.0	50.0	58.1	57.3	645-1075 bp	1.0	86.0
infA_p4	ATTCGTCGTCGTTTTCGGAT	AATGACAGACCGAGAAGCTC	45.0	50.0	58.0	57.3	223-659 bp	0.5	66.6
infA_p5	ATTCGTCGTCGTTTTCGGAT	AAAACAAGTTAGCCGTCCCG	45.0	50.0	58.0	58.8	328-764 bp	0.5	65.8