Hotspot

ccsA-psaC

SSC · 94480-94771 · 292 bp

Back to Taxillus

Pi 0.081

MarkerSeek score 70.40

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 292
variable_sites: 89
pi: 0.081
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	GAGCTGTGTGGGCTAATGAA	CATGTATCGGGTGCACTCAA	50.0	50.0	57.9	58.0	708-754 bp	1.0	90.9
ccsA-psaC_p2	GAGCTGTGTGGGCTAATGAA	TGTATCGGGTGCACTCAATG	50.0	50.0	57.9	58.0	706-752 bp	1.0	90.9
ccsA-psaC_p3	GAGCTGTGTGGGCTAATGAA	GTATCGGGTGCACTCAATGT	50.0	50.0	57.9	58.0	705-751 bp	1.0	90.9
ccsA-psaC_p4	GAGCTGTGTGGGCTAATGAA	CAAATTGCTTCTGCTCCACG	50.0	50.0	57.9	58.0	625-671 bp	1.0	90.9
ccsA-psaC_p5	AGCTGTGTGGGCTAATGAAG	TGTATCGGGTGCACTCAATG	50.0	50.0	57.9	58.0	705-751 bp	1.0	90.9