Hotspot

atpB-rbcL

LSC · 43002-43678 · 677 bp

Back to Taxillus

Pi 0.0301

MarkerSeek score 87.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 78
pi: 0.0301
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TACATCCAATACCGGACCGA	GCTTTGAATCCAACACCTGC	50.0	50.0	57.9	57.9	792-937 bp	0.928571	86.4
atpB-rbcL_p2	CGGACCGATGATTTGATCGA	GCTTTGAATCCAACACCTGC	50.0	50.0	57.9	57.9	780-925 bp	0.928571	86.0
atpB-rbcL_p3	TCAGGCACAGAAAACCCAAA	GCTTTGAATCCAACACCTGC	45.0	50.0	57.8	57.9	740-885 bp	0.928571	86.0
atpB-rbcL_p4	TTCAGGCACAGAAAACCCAA	GCTTTGAATCCAACACCTGC	45.0	50.0	57.8	57.9	741-886 bp	0.857143	83.0
atpB-rbcL_p5	TTTCAGGCACAGAAAACCCA	GCTTTGAATCCAACACCTGC	45.0	50.0	57.8	57.9	742-887 bp	0.857143	83.0