Hotspot

trnS-UGA-psbZ

LSC · 28615-28989 · 375 bp

Back to Taxillus

Pi 0.0425

MarkerSeek score 79.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 57
pi: 0.0425
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CGTCGGGAGAAGCAAATACA	55.0	50.0	58.0	58.0	452-548 bp	1.0	91.8
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	CGTCGGGAGAAGCAAATACA	55.0	50.0	58.1	58.0	459-555 bp	1.0	91.5
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	CGTCGGGAGAAGCAAATACA	55.0	50.0	58.1	58.0	460-556 bp	1.0	91.5
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	CGTCGGGAGAAGCAAATACA	55.0	50.0	58.1	58.0	467-563 bp	1.0	91.5
trnS-UGA-psbZ_p5	TCGAACCCCCGATAGTTCTT	CGTCGGGAGAAGCAAATACA	50.0	50.0	58.1	58.0	509-605 bp	1.0	90.8