Hotspot

trnE-UUC-trnT-GGU

LSC · 24455-24856 · 402 bp

Back to Taxillus

Pi 0.0467

MarkerSeek score 76.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 402
variable_sites: 64
pi: 0.0467
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	500-550 bp	1.0	89.8
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	479-529 bp	1.0	89.7
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	497-547 bp	1.0	88.7
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	476-526 bp	1.0	88.5
trnE-UUC-trnT-GGU_p5	GCTGCCTCCTTGAAAGAGAG	TTGAACCGATGACTTACGCC	55.0	50.0	58.3	58.3	502-552 bp	1.0	87.6