Hotspot

trnD-GUC-trnY-GUA

LSC · 23941-24256 · 316 bp

Back to Taxillus

Pi 0.038

MarkerSeek score 76.60

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 316
variable_sites: 39
pi: 0.038
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTAACCAGTTGAA	TCTACGCTGGTTCAAATCCG	50.0	50.0	57.8	58.0	360-470 bp	1.0	90.3
trnD-GUC-trnY-GUA_p2	TTTATTGGCTTCGTCGGGAC	TCTACGCTGGTTCAAATCCG	50.0	50.0	58.3	58.0	418-528 bp	1.0	88.6
trnD-GUC-trnY-GUA_p3	TCCACATACGACTCCCTACC	TCTACGCTGGTTCAAATCCG	55.0	50.0	57.9	58.0	462-660 bp	0.5	68.6
trnD-GUC-trnY-GUA_p4	CCACATACGACTCCCTACCA	TCTACGCTGGTTCAAATCCG	55.0	50.0	58.2	58.0	461-659 bp	0.5	67.3
trnD-GUC-trnY-GUA_p5	TCCACATACGACTCCCTACC	CGTTGGCAATTTGTCTACGC	55.0	50.0	57.9	58.4	475-673 bp	0.5	65.4