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trnC-GCA-petN

LSC · 22255-22542 · 288 bp

Back to Taxillus

Pi 0.0473

MarkerSeek score 83.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 288
variable_sites: 45
pi: 0.0473
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAT	50.0	45.0	58.1	58.2	246-376 bp	1.0	89.5
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TAAAGCAGCCCAAGCGATAC	50.0	50.0	58.1	58.3	244-374 bp	1.0	88.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCGATA	50.0	45.0	58.1	57.6	245-375 bp	1.0	88.0
trnC-GCA-petN_p4	GTATCATTTTGGCGGCATGG	ATTAAAGCAGCCCAAGCGAT	50.0	45.0	57.9	58.2	298-428 bp	0.928571	86.2
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	TAAAGCAGCCCAAGCGATAC	50.0	50.0	57.9	58.3	296-426 bp	0.928571	85.3