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rps2-rpoC2

LSC · 10925-11122 · 198 bp

Back to Taxillus

Pi 0.0427

MarkerSeek score 76.90

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 198
variable_sites: 31
pi: 0.0427
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GGTGCCATTCTAGGATTCCA	ACGATTCTCAGAAGATTAGGA	50.0	38.1	57.0	53.5	322 bp	0.071429	2.9
rps2-rpoC2_p2	GGTGCCATTCTAGGATTCCA	AACGATTCTCAGAAGATTAGGA	50.0	36.4	57.0	54.4	323 bp	0.071429	2.9
rps2-rpoC2_p3	AGGTGCCATTCTAGGATTCC	ACGATTCTCAGAAGATTAGGA	50.0	38.1	56.7	53.5	323 bp	0.071429	2.9
rps2-rpoC2_p4	GGTGCCATTCTAGGATTCCA	AAACGATTCTCAGAAGATTAGGA	50.0	34.8	57.0	55.2	324 bp	0.071429	2.9
rps2-rpoC2_p5	GGTGCCATTCTAGGATTCCA	ACGATTCTCAGAAGATTAGGAT	50.0	36.4	57.0	54.2	322 bp	0.071429	2.9