Hotspot

psbA-matK

LSC · 1249-1809 · 561 bp

Back to Taxillus

Pi 0.0308

MarkerSeek score 75.50

Primer available yes

Label psbA-matK

Feature Metadata

label_name: psbA-matK
feature_type: igs
region: LSC
length_bp: 561
variable_sites: 65
pi: 0.0308
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	GCTTTCACTTGTGCGTCTCT	ACGATCTAGTCAATCATTCATGA	50.0	34.8	58.8	55.3	617-633 bp	0.928571	48.1
psbA-matK_p2	GCTTTCACTTGTGCGTCTCT	TCAACGATCTAGTCAATCATTCA	50.0	34.8	58.8	55.5	620-636 bp	0.928571	48.0
psbA-matK_p3	TTTCACTTGTGCGTCTCTCT	TCAACGATCTAGTCAATCATTCA	45.0	34.8	57.1	55.5	618-634 bp	0.928571	48.0
psbA-matK_p4	GCTTTCACTTGTGCGTCTCT	CAACGATCTAGTCAATCATTCATGA	50.0	36.0	58.8	57.3	619-635 bp	0.928571	48.0
psbA-matK_p5	GCTTTCACTTGTGCGTCTCT	TCAACGATCTAGTCAATCATTCATG	50.0	36.0	58.8	57.3	620-636 bp	0.928571	48.0