Hotspot

ndhD-psaC

SSC · 119005-119146 · 142 bp

Back to Tashiroea

Pi 0.0312

MarkerSeek score 57.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 142
variable_sites: 9
pi: 0.0312
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TGTTAACCAAGGAAAATAATTCGT	AAACAACTCGAAGCATGGGT	29.2	45.0	55.3	58.0	196-198 bp	1.0	44.5
ndhD-psaC_p2	TGTTAACCAAGGAAAATAATTCGT	GGCATGAAACAACTCGAAGC	29.2	50.0	55.3	58.0	202-204 bp	1.0	44.3
ndhD-psaC_p3	ACCTACAATTATTGTTAACCAAGGA	AAACAACTCGAAGCATGGGT	32.0	45.0	56.4	58.0	208-210 bp	1.0	44.2
ndhD-psaC_p4	ACCTACAATTATTGTTAACCAAGGA	GGCATGAAACAACTCGAAGC	32.0	50.0	56.4	58.0	214-216 bp	1.0	44.1
ndhD-psaC_p5	ACCTACAATTATTGTTAACCAAGGA	AGAGATGTGAATCCGCTTGC	32.0	50.0	56.4	58.3	268-270 bp	1.0	43.3