Hotspot

ndhF-rpl32

SSC · 114671-115605 · 935 bp

Back to Tashiroea

Pi 0.0253

MarkerSeek score 67.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 935
variable_sites: 55
pi: 0.0253
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGCAACTGCACCTGTAAAT	GCAGCGTGTTTACCGATTTC	45.0	50.0	58.0	58.1	2100-2143 bp	1.0	85.7
ndhF-rpl32_p2	TGGCAACTGCACCTGTAAAT	GATTTCACCATAGCGGCGTA	45.0	50.0	58.0	58.1	2086-2129 bp	1.0	85.6
ndhF-rpl32_p3	CGCCTACACGATTCGTTACA	GCAGCGTGTTTACCGATTTC	50.0	50.0	58.1	58.1	1938-1972 bp	0.833333	78.3
ndhF-rpl32_p4	TGGCAACTGCACCTGTAAAT	CTTCCTAAGAGCAGCGTGTT	45.0	50.0	58.0	57.9	2144 bp	0.166667	45.9
ndhF-rpl32_p5	CGCCTACACGATTCGTTACA	CTTCCTAAGAGCAGCGTGTT	50.0	50.0	58.1	57.9	1982 bp	0.166667	45.2