Hotspot

accD-psaI

LSC · 59819-60665 · 847 bp

Back to Tashiroea

Pi 0.0205

MarkerSeek score 58.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 37
pi: 0.0205
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACAGTACCCGAGGATTCACA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	994-1055 bp	1.0	86.3
accD-psaI_p2	CGATTGATCCAGGGACTTGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1527-1588 bp	1.0	85.8
accD-psaI_p3	CGATTGATCCAGGGACTTGG	ATCGACTTTCCCTTTCAGGC	55.0	50.0	58.0	57.9	1798-1857 bp	1.0	85.0
accD-psaI_p4	CGATTGATCCAGGGACTTGG	GTGCCGAACCCTTTCCTTTA	55.0	50.0	58.0	58.1	1974 bp	0.166667	45.5
accD-psaI_p5	CGATTGATCCAGGGACTTGG	CAACTAGTGCCGAACCCTTT	55.0	50.0	58.0	58.1	1980 bp	0.166667	45.5