Hotspot

rbcL-accD

LSC · 57714-58342 · 629 bp

Back to Tashiroea

Pi 0.0132

MarkerSeek score 60.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 16
pi: 0.0132
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AAGCTAGCAAATGGAGTCCG	ACATCGAATTAAACCGCCGT	50.0	45.0	58.0	58.3	721-746 bp	1.0	83.0
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	ACATCGAATTAAACCGCCGT	50.0	45.0	57.9	58.3	692-717 bp	1.0	82.5
rbcL-accD_p3	AATGGAGTCCGGAACTAGCT	ACATCGAATTAAACCGCCGT	50.0	45.0	58.1	58.3	712-737 bp	1.0	82.2
rbcL-accD_p4	AAGCTAGCAAATGGAGTCCG	ATCCTCACTTCCACGAGTGT	50.0	50.0	58.0	58.4	810-835 bp	1.0	81.6
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	ATCCTCACTTCCACGAGTGT	50.0	50.0	57.9	58.4	781-806 bp	1.0	81.1