Pi
0.0105
MarkerSeek score
67.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 742-870 bp | 1.0 | 84.9 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 750-878 bp | 1.0 | 84.4 |
| trnT-UGU-trnL-UAA_p3 | TAGACAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 793-921 bp | 1.0 | 84.1 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 774-902 bp | 1.0 | 84.0 |
| trnT-UGU-trnL-UAA_p5 | TAGACAAAAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 801-929 bp | 1.0 | 83.5 |