Hotspot

rps16-trnQ-UUG

LSC · 5720-6740 · 1021 bp

Back to Tashiroea

Pi 0.0191
MarkerSeek score 62.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1021
variable_sites
40
pi
0.0191
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGAAAATGGCAGCAACATGC TAGAGCAGCTTGCCAAACAA 45.0 45.0 58.2 58.0 1896-1968 bp 1.0 84.8
rps16-trnQ-UUG_p2 TGAAAATGGCAGCAACATGC TTAGAGCAGCTTGCCAAACA 45.0 45.0 58.2 58.0 1897-1969 bp 1.0 84.8
rps16-trnQ-UUG_p3 AACATGCCCCCTTTTATGCT TAGAGCAGCTTGCCAAACAA 45.0 45.0 57.7 58.0 1883-1955 bp 0.833333 77.1
rps16-trnQ-UUG_p4 ACATGCCCCCTTTTATGCTT TAGAGCAGCTTGCCAAACAA 45.0 45.0 57.7 58.0 1882-1954 bp 0.833333 77.1
rps16-trnQ-UUG_p5 ACATGCCCCCTTTTATGCTT TTAGAGCAGCTTGCCAAACA 45.0 45.0 57.7 58.0 1883-1955 bp 0.833333 77.1