Hotspot

ycf1

SSC · 122440-127494 · 5055 bp

Back to Taraxacum

Pi 0.00593

MarkerSeek score 40.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5055
variable_sites: 75
pi: 0.00593
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACCTTCTAAAAATTGATTGAGTT	GAAATCCTCATTCCCCGCTT	26.1	50.0	52.5	57.9	258 bp	1.0	41.9
ycf1_p2	ACCTTCTAAAAATTGATTGAGTTAGT	GAAATCCTCATTCCCCGCTT	26.9	50.0	55.4	57.9	258 bp	1.0	41.9
ycf1_p3	ACCTTCTAAAAATTGATTGAGTT	AAGAAATCCTCATTCCCCGC	26.1	50.0	52.5	57.9	260 bp	1.0	41.8
ycf1_p4	ACATACCTTCTAAAAATTGATTGAGT	AAGAAATCCTCATTCCCCGC	26.9	50.0	55.4	57.9	264 bp	1.0	41.8
ycf1_p5	ACATACCTTCTAAAAATTGATTGAGT	GAAATCCTCATTCCCCGCTT	26.9	50.0	55.4	57.9	262 bp	1.0	41.8