Hotspot

ccsA

SSC · 113006-113980 · 975 bp

Back to Taraxacum

Pi 0.00176

MarkerSeek score 36.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 975
variable_sites: 6
pi: 0.00176
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1520-1578 bp	1.0	83.9
ccsA_p2	TGAAATCGGTAGACACGCTG	GTTTTTGATTCTGGACCGCG	50.0	50.0	58.0	58.0	1519-1577 bp	1.0	83.9
ccsA_p3	GTGAAATCGGTAGACACGCT	ATGCTTGGTCTTTGCCAGAA	50.0	45.0	58.0	58.0	1252-1291 bp	1.0	83.5
ccsA_p4	TGAAATCGGTAGACACGCTG	ATGCTTGGTCTTTGCCAGAA	50.0	45.0	58.0	58.0	1251-1290 bp	1.0	83.5
ccsA_p5	GTGAAATCGGTAGACACGCT	GCTTGCATTACCGGGTATGA	50.0	50.0	58.0	58.0	1725-1783 bp	1.0	83.5