Hotspot

ccsA-ndhD

SSC · 113981-114254 · 274 bp

Back to Taraxacum

Pi 0.0247

MarkerSeek score 60.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 274
variable_sites: 15
pi: 0.0247
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGCTATTTTGGGGTGAACCT	TCAGTTGACAAGGTTGAAGGT	45.0	42.9	57.0	57.4	372-394 bp	1.0	66.1
ccsA-ndhD_p2	ATGCTATTTTGGGGTGAACCT	TCAGTTGACAAGGTTGAAGGT	42.9	42.9	57.5	57.4	373-395 bp	1.0	62.2
ccsA-ndhD_p3	TGCTATTTTGGGGTGAACCT	ATCAGTTGACAAGGTTGAAGGT	45.0	40.9	57.0	57.8	373-395 bp	1.0	61.9
ccsA-ndhD_p4	TGCTATTTTGGGGTGAACCT	CAGTTGACAAGGTTGAAGGT	45.0	45.0	57.0	55.8	371-393 bp	1.0	61.6
ccsA-ndhD_p5	TGCTATTTTGGGGTGAACCT	TCAGTTGACAAGGTTGAAGG	45.0	45.0	57.0	55.5	372-394 bp	1.0	59.3