Hotspot

rpl32-trnL-UAG

SSC · 111933-112811 · 879 bp

Back to Taraxacum

Pi 0.00758
MarkerSeek score 52.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
879
variable_sites
14
pi
0.00758
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCGGCATTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 982-996 bp 1.0 81.6
rpl32-trnL-UAG_p2 GGCGGCATTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 983-997 bp 1.0 81.6
rpl32-trnL-UAG_p3 AAAGGATATTGGGCGGCATT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 993-1007 bp 1.0 81.5
rpl32-trnL-UAG_p4 AAAGGATATTGGGCGGCATT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 994-1008 bp 1.0 81.5
rpl32-trnL-UAG_p5 GGCGGCATTAAAAGCTTTGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.9 57.9 992-1006 bp 1.0 80.6