Hotspot

ndhF-rpl32

SSC · 110692-111767 · 1076 bp

Back to Taraxacum

Pi 0.00965

MarkerSeek score 53.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1076
variable_sites: 28
pi: 0.00965
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGGAAAGGT	ACAAAGCTTTTAATGCCGCC	50.0	45.0	57.0	57.9	1221-1229 bp	1.0	73.8
ndhF-rpl32_p2	CTGGAAGTGGAAGGAAAGGT	AATGCCGCCCAATATCCTTT	50.0	45.0	57.0	57.9	1210-1218 bp	1.0	73.7
ndhF-rpl32_p3	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTT	50.0	50.0	57.0	57.6	1206-1214 bp	1.0	71.5
ndhF-rpl32_p4	CTGGAAGTGGAAGGAAAGGT	ATGCCGCCCAATATCCTTTC	50.0	50.0	57.0	58.4	1209-1217 bp	1.0	71.4
ndhF-rpl32_p5	CTGGAAGTGGAAGGAAAGGT	CAAAGCTTTTAATGCCGCCC	50.0	50.0	57.0	58.6	1220-1228 bp	1.0	69.3