Hotspot

trnL-UAA-trnF-GAA

LSC · 47325-47680 · 356 bp

Back to Taraxacum

Pi 0.0203

MarkerSeek score 64.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 356
variable_sites: 14
pi: 0.0203
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	ACATGTCAATACCGGCAACA	TTTGAACTGGTGACACGAGG	45.0	50.0	57.8	58.1	471-547 bp	1.0	84.3
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	412-488 bp	1.0	82.3
trnL-UAA-trnF-GAA_p3	ACATGTCAATACCGGCAACA	GAACTGGTGACACGAGGATT	45.0	50.0	57.8	57.5	468-544 bp	1.0	81.1
trnL-UAA-trnF-GAA_p4	ACATGTCAATACCGGCAACA	TCCTCTGCTCTACCAACTGA	45.0	50.0	57.8	57.4	366-474 bp	1.0	80.2
trnL-UAA-trnF-GAA_p5	ACATGTCAATACCGGCAACA	TCAGTCCTCTGCTCTACCAA	45.0	50.0	57.8	57.4	447-523 bp	1.0	80.0