Hotspot

trnK-UUU-rps16

LSC · 4390-5151 · 762 bp

Back to Taraxacum

Pi 0.00577
MarkerSeek score 55.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
762
variable_sites
9
pi
0.00577
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CCTTGAAAAAGGTGCTCAGC 50.0 50.0 58.2 57.6 864-911 bp 1.0 79.5
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT CTTGAAAAAGGTGCTCAGCC 50.0 50.0 58.2 57.6 863-910 bp 1.0 79.5
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT CCTTGAAAAAGGTGCTCAGC 50.0 50.0 58.2 57.6 863-910 bp 1.0 79.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT CTTGAAAAAGGTGCTCAGCC 50.0 50.0 58.2 57.6 862-909 bp 1.0 79.5
trnK-UUU-rps16_p5 TCCTAGCCGCACTTAAAAGC CTTGAAAAAGGTGCTCAGCC 50.0 50.0 58.3 57.6 878-925 bp 1.0 78.7