Hotspot

rpoA

LSC · 79446-80453 · 1008 bp

Back to Tapeinosperma

Pi 0.00198

MarkerSeek score 59.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1008
variable_sites: 2
pi: 0.00198
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GGATCGAGTCCAGATTTATAT	TGGCTGTAGACCCCCTAAAA	38.1	50.0	52.0	58.0	1213 bp	1.0	40.1
rpoA_p2	GGATCGAGTCCAGATTTATAT	AATGGCTGTAGACCCCCTAA	38.1	50.0	52.0	57.7	1215 bp	1.0	40.1
rpoA_p3	GGATCGAGTCCAGATTTATAT	ATGGCTGTAGACCCCCTAAA	38.1	50.0	52.0	57.7	1214 bp	1.0	40.1
rpoA_p4	GGATCGAGTCCAGATTTATAT	ATAATGGCTGTAGACCCCCT	38.1	50.0	52.0	57.5	1217 bp	1.0	40.1
rpoA_p5	GGATCGAGTCCAGATTTATAT	CATAATGGCTGTAGACCCCC	38.1	55.0	52.0	57.4	1218 bp	1.0	40.1