Hotspot

petB

LSC · 76328-77734 · 1407 bp

Back to Tapeinosperma

Pi 0.00284

MarkerSeek score 60.40

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1407
variable_sites: 4
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GCAGTTTGACCGTGGAATTT	GTTCCTCCCAAACGTGCTAA	45.0	50.0	57.2	58.1	1506 bp	1.0	73.0
petB_p2	GGCAGTTTGACCGTGGAATT	GTTCCTCCCAAACGTGCTAA	50.0	50.0	59.0	58.1	1507 bp	1.0	71.0
petB_p3	GCAGTTTGACCGTGGAATTT	TTCCTCCCAAACGTGCTAAA	45.0	45.0	57.2	57.4	1505 bp	1.0	68.7
petB_p4	ATTTGGCAGTTTGACCGTGG	GTTCCTCCCAAACGTGCTAA	50.0	50.0	59.3	58.1	1511 bp	1.0	68.7
petB_p5	GCAGTTTGACCGTGGAATTTC	GTTCCTCCCAAACGTGCTAA	47.6	50.0	58.3	58.1	1506 bp	1.0	68.6