Hotspot

ccsA

SSC · 115571-116548 · 978 bp

Back to Tapeinosperma

Pi 0.00102

MarkerSeek score 51.50

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGGCATGCCCTCTTCTAAAA	TTCATATGGTTCTCGCCGAC	50.0	50.0	58.2	57.8	1176 bp	1.0	76.3
ccsA_p2	CGGCATGCCCTCTTCTAAAA	CATATGGTTCTCGCCGACTT	50.0	50.0	58.2	57.8	1174 bp	1.0	76.3
ccsA_p3	CGGCATGCCCTCTTCTAAAA	GTTCTCGCCGACTTACACAA	50.0	50.0	58.2	58.2	1168 bp	1.0	76.3
ccsA_p4	CGGCATGCCCTCTTCTAAAA	TTCTCGCCGACTTACACAAA	50.0	45.0	58.2	57.5	1167 bp	1.0	74.0
ccsA_p5	CGGCATGCCCTCTTCTAAAA	TCATATGGTTCTCGCCGACT	50.0	50.0	58.2	58.6	1175 bp	1.0	73.3