Hotspot

rpl20-rps12

LSC · 70207-70996 · 790 bp

Back to Tapeinosperma

Pi 0.00127

MarkerSeek score 59.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 1
pi: 0.00127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGCTCCTCGAAAGC	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	943-944 bp	1.0	75.7
rpl20-rps12_p2	TGAATGAGCTCCTCGAAAGC	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	906-907 bp	1.0	74.2
rpl20-rps12_p3	TCCCCGTCTAATTCTGGTCA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.8	58.5	877-878 bp	1.0	73.9
rpl20-rps12_p4	TGAATGAGCTCCTCGAAAGC	AGCGTCGAGGAACATGTACT	50.0	50.0	58.0	58.8	905-906 bp	1.0	73.4
rpl20-rps12_p5	TGAATGAGCTCCTCGAAAGC	TCAGCGTCGAGGAACATGTA	50.0	50.0	58.0	58.8	907-908 bp	1.0	73.4