Hotspot

ycf4-cemA

LSC · 61398-62132 · 735 bp

Back to Tapeinosperma

Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 2
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGACGCGCACTGATGATAA	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.0	57.6	903 bp	1.0	77.0
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	AGAAATCCACCAGGGCAAAA	45.0	45.0	58.1	57.6	846 bp	1.0	76.2
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	AGAAATCCACCAGGGCAAAA	45.0	45.0	57.8	57.6	862 bp	1.0	75.8
ycf4-cemA_p4	TGACGCGCACTGATGATAAT	AGAAATCCACCAGGGCAAAA	45.0	45.0	57.8	57.6	902 bp	1.0	75.2
ycf4-cemA_p5	TGCGTGTACCAATTGAGGTT	AGAAATCCACCAGGGCAAAA	45.0	45.0	57.7	57.6	833 bp	1.0	74.9